
Types of Stem Cell
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Hematopoietic stem cells / mononuclear stem cells are isolated from the blood or bone marrow or umbilical cord blood.
The proportion of Hematopoietic stem cells is around 0.01% in bone marrow and 0.001% in blood. They can differentiate in to a variety of specialized cells and have capacity of self renewal. They are programmed in such a way that they self destruct themselves if unneeded or harmful, called apoptosis. It is consider that there are two types of Hematopoietic stem cells, one which can renew themselves for long term and proportion of this kind of cells is around 0.001% in bone marrow. The other types of cells are progenitor or precursor cells which can not renew themselves over a long term. They are immature cells which are precursor to fully differentiated cells of the same type of tissue like blood cells, immune cells, blood vessels, muscle cells, bone etc. and hence bone marrow play a vital role in maintaining health. Therefore BMNC (bone marrow mononuclear cells) transplantation is used to treat blood cancers and autoimmune diseases and also it may represent a novel therapeutic option for treating some of the diseases like Cardiovascular Diseases, Spinal Cord Injury etc.
At StemCure mononuclear cells are isolated from its various sources and further used for differentiation into specific types of tissue for clinical applications or drug study. Analytical tests are performed before and after Isolation of mononuclear cells to ensure its quality standards.

Endothelial progenitor cells are rare cells that circulate in the blood with the ability to differentiate into endothelial cells, the cells that make up the lining of blood vessels. The process by which blood vessels are born de novo is known as vasculogenesis. Some study suggests that endothelial injury in the absence of sufficient circulating progenitor cells may affect the progression of cardiovascular disease.
Endothelial progenitor cells can applicable for cardiovascular disorders like dilated cardiomyopathy, heart failure, myocardial infarction, coronary artery disorder, primary pulmonary hypertension.
At StemCure we provide cultured endothelial progenitor cells (EPCs). We are successfully culturing EPCs from BM/ PB samples since 2006. At StemCure we isolate and culture EPCs from Bone marrow or peripheral blood by following GMP / GTP standards and maintaining aseptic conditions. After expansion the cultured cells are confirmed by phenotypic and genotypic confirmation like morphological analysis, surface marker study and gene expression study. In gene expression study, we confirm the expression of marker specific to differentiation of EPCs and also check for other markers. Which are specific for MNC to rule out presence or absence of other types of cells. More over the final product is assess for some of the parentral drug criteria like sterility and endotoxin level detections. After confirmation, the cultured cells are delivered to the clinicians for application. Normally the culture procedure takes approximately 7-10 days, than it depends on source sample and number of cells required.
http://www.nejm.org/doi/full/10.1056/NEJMoa022287


Mesenchymal stem cells can applicable in various disorders like orthopedic disorders, diabetes, interstitial lung disease, spinal cord injury, skin disorders, cosmetic therapies.
At StemCure we isolate and culture MSCs from bone marrow, adipose tissue and umbilical cord by following GMP / GTP standards and maintaining aseptic conditions. After expansion the cultured cells are confirmed by phenotypic and genotypic confirmation like morphological analysis, surface marker study and gene expression study. In gene expression study, we confirm the expression of marker specific to differentiation of MSCs and also check for other markers, which are specific for MNC to rule out presence or absence of other types of cells. More over the final product is assess for some of the parentral drug criteria like sterility and endotoxin level detections. After confirmation, the cultured cells are delivered to the clinicians for application. Normally the entire culture procedure takes approximately 20 days to 1 month than it depends on source of cells and number of cells required.
References:
http://www.hindawi.com/journals/sci/2010/215625/
Cartilage biopsy, bone marrow and mesenchymal stem cells are the sources for chondrocytes.

Chondrocytes are applicable in cartilage defects like Osteochondritis Dissecans (OCD), Cartilage defects like cartilage Fracture, Sports Injury.
At StemCure we isolate and culture Chondrocytes from cartilage biopsy by following GMP/GTP standards and maintaining aseptic conditions. Chondrocytes are isolated by enzymatic digestion and cultivated in specific growth media. After expansion the cultured cells are lifted from the tissue culture surface and cell suspension is prepared for transplantation. All necessary test like endotoxins, sterility etc. are performed before dispatch of the suspension. After confirmation, the cultured cells are delivered to the clinicians for an application. The entire culture procedure takes approximately 1 month.


Melanocytes are melanin-producing cells located in the bottom layer (the stratum basal) of the skin's epidermis, Melanin is a pigment that is responsible primarily for the color of skin and also protects skin form hazardous UVa and UVb rays by absorbing them. Hence the role of active melanocytes is also important to save the skin from being affected by skin cancer.
Melanocytes are used for the treatment of depigmentation like stable vitiligo.
At StemCure we isolate and culture Melanocyte from autologus skin biopsy by following GMP/GTP standards and maintaining aseptic conditions. A small piece of the patient’s own normal skin from the non-exposed area, usually from the back of the ear lobe or buttocks (which is approximately 1/10th of the diseased patch), is taken by Skin specialist / surgeon / cosmetic surgeon at their facility in the transport media supplied in the kit. The skin sample is digested and melanocytes are isolated and cultured in our special cell culture laboratory using different media. There are two products of the melanocytes for depigmentation, melanocytes and keratinocytes cell suspension and cultured melanocytes suspension. The preference of the product is largely based on the area of the patch and other consideration taken by the clinician.

Skin biopsy is the source for keratinocytes.
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Cultured Keratinocytes are used for the treatment of Burns, Wounds and Skin ulcers.
At StemCure we isolate and culture Keratinocyte from autologus skin biopsy by following GMP/GTP standards and maintaining aseptic conditions A small piece of the patient’s own normal skin from the non-exposed area, usually from the back of the ear lobe or buttocks (which is approximately 1/10th of the diseased patch), is taken by Skin specialist / surgeon / cosmetic surgeon at their facility in the transport media supplied in the kit. The skin sample is digested and keratinocytes are isolated and cultured in our state of art cell culture laboratory using specified media. Once the desired confluency is achieved, then cells are prepared for transplantation. The duration of the culture process depends upon area to be treated and size of the biopsy.